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Basic Science Workshop
Saturday, April 10, 2010
Chair: Kate Loveland, PhD
Location: Regency C
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| SATURDAY, APRIL 10, 2010 |
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| 7:00 a.m. – 7:00 p.m. |
Registration/Information Desk Open
Location: Regency Foyer |
| 4:00 p.m. – 9:30 p.m. |
Exhibit Hall Open
Location: Colonnade Salon |
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| 8:30 a.m. – 8:35 a.m. |
Welcome and Introduction
Kate Loveland, PhD
Monash University, Australia |
| 8:35 a.m. – 10:30 a.m. |
Cell Type Identification Strategies
Convenor: Sarah Meachem, PhD
Prince Henry’s Institute, Australia
Sarah Meachem, PhD
Poor vs good histological procedures, from different mediums to fixatives. Experiences with a range of species, from rodent to human tissue. Different staining methods and best practice stereological techniques. Guide to easy staging of tubules. Markers of tight junctions, cell cycle and apoptosis in histological sections
Sarah Kimmins, PhD
Markers for identification and quantification of germ and somatic cells in the testis at different stages of development
Catherine Itman, PhD
Real time PCR: Key factors in design and analysis
Kate Loveland, PhD
Antibodies, in situ hybridization probes and FACS strategies we use to analyze germ and somatic cells
Beth Snyder
In situ hybridization; LacZ detection; Microarray data analysis
Marv Meistrich, PhD
Cell type identification in smears
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| 10:30 a.m. – 10:40 a.m. |
Break |
| 10:40 a.m. – 11:00 a.m. |
Delegate Exchange |
| 11:00 a.m. – 1:00 p.m. |
Cell Separation and Culture Approaches
Convenor: Marie-Claude Hofmann, PhD
University of Illinois, Urbana
Marie-Claude Hofmann, PhD
Spermatogonial stem cell isolation and analysis in vitro
Thomas Garcia, PhD
Reporter mice for stem cell isolation
Marv Meistrich, PhD
Cell isolation using Staput gradients and centrifugal elutriator/ Percoll gradients
Marv Meistrich, PhD; Wei Zhou, PhD
Analysis of spermatogonial stem cells in vivo
Wei Zhou, PhD
MACs and cell adhesion molecules
Catherine Itman, PhD
Sertoli germ cell culture and analyses including signalling pathway activation
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| 1:00 p.m. – 1:30 p.m. |
Lunch
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| 1:30 p.m. – 3:30 p.m. |
Function/Fertility Assessments
Convenor: Moira O’Bryan, PhD
Monash University, Australia
Moira O’Bryan, PhD
Phenotype assessment for infertile mouse lines
Carol Linder, PhD
Variant 129 mouse strains: an important issue in reproduction and genetics
Phil Purdy, PhD
Flow cytometric analyses (plasma membrane integrity, calcium content, acrosomal integrity, membrane fluidity, etc.) on numerous species; testis and sperm cryopreservation; CASA
Gabriela Mendeluk, PhD
Biophysical methods to study sperm function
Janice Bailey, PhD
Benchtop flow cytometry application for semen analysis
Wendie A. Robbins, PhD
Epidemiological approaches to understanding male fertility/infertility
Ronald Wiehle, PhD
Conducing fertility analyses with Repros Therapeutics product
Vincent Tsai, MD
Human sperm function device
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| 3:30 p.m. – 4:00 p.m. |
Delegate Exchange |
| 4:00 p.m. |
Adjourn
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